Efficient ¹⁵N-hyperpolarization of [¹⁵N₃]metronidazole was reported previously using the Signal Amplification By Reversible Exchange in SHield Enabled Alignment Transfer (SABRE-SHEATH) technique. This hyperpolarized FDA-approved antibiotic is a potential contrast agent because it can be administered in a large dose and because previous studies revealed long-lasting HP states with exponential decay constant T₁ values of up to 10 min. Possible hypoxia-sensing applications have been proposed using hyperpolarized [¹⁵N₃]metronidazole. In this work, we report on the functionalization of [¹⁵N₃]metronidazole with a fluorine-19 moiety via a one-step reaction to substitute the −OH group. SABRE-SHEATH hyperpolarization studies of fluoro-[¹⁵N₃]metronidazole revealed efficient hyperpolarization of all three ¹⁵N sites with maximum %P_15N values ranging from 4.2 to 6.2%, indicating efficient spin-relayed polarization transfer in microtesla fields via the network formed by ²J_15N-15N. The corresponding ¹⁵N to ¹⁹F spin-relayed polarization transfer was found to be far less efficient with %P_19F of 0.16%, i.e., more than an order of magnitude lower than that of ¹⁵N. Relaxation dynamics studies in microtesla fields support a spin-relayed polarization transfer mechanism because all ¹⁵N and ¹⁹F spins share the same T₁ value of ca. 16–20 s and the same magnetic field profile for the SABRE-SHEATH polarization process. We envision the use of fluoro-[¹⁵N₃]metronidazole as a potential hypoxia sensor. It is anticipated that under hypoxic conditions, the nitro group of fluoro-[¹⁵N₃]metronidazole undergoes electronic stepwise reduction to an amino derivative. Ab initio calculations of ¹⁵N and ¹⁹F chemical shifts of fluoro-[¹⁵N₃]metronidazole and its putative hypoxia-induced metabolites clearly indicate that the chemical shift dispersions of all three ¹⁵N sites and the ¹⁹F site are large enough to enable the envisioned hypoxia-sensing approaches.