Layered nanocarriers are polymeric systems integrated with layers of glucose-responsive polymers and polyethylene glycol monomethyl ether 2000 PEG₂₀₀₀ to enhance the targeting effect and release kinetics. The prime purpose of this research study is to demonstrate a controlled release of Quercetin from formulation of quercetin (Qu)-based layered nanocarriers (LNCs) with higher stability and anti-diabetic activity. The QuLNCs are synthesized using the nanoprecipitation method and optimized by Box-Behnken design of the Design of Experiment (DoE) method. The Quercetin Nanoparticles (QuNPs) are prepared using Polyvinyl alcohol (PVA) and Poly (D,L- lactic-co-glycolic acid) (PLGA) polymers where the two layers of Phenylboronic acid (PBA) are conjugated using 3-amino propyl-triethoxysilane (APTES) as a functionalizing agent followed by PEGylation of the entire system using PEG₂₀₀₀. The optimized QuLNCs are characterized by various parameters like Particle size (PS), Zeta potential (ZP), % Entrapment efficiency (%EE), Attenuated total reflectance- Fourier Transform Infrared Spectroscopy (ATR-FTIR), Differential scanning Calorimetry (DSC), Transmission electron microscopy (TEM), in vitro as well as in vivo studies. The QuLNCs showed a % entrapment efficiency of 82.846 ± 0.957% and release of 85.04 ± 3.21% of Qu for 24 h from the layered nanocarriers. The in vivo studies of QuLNCs exhibited a significant controlled release of quercetin for modulating blood glucose levels. Hence, these results proved QuLNCs system acts as a favorable approach for the treatment of type 1 diabetes to offer a longer duration of action.