523. A New Fluorene–Based Fluorescent Probe for Recognition of Hypochlorite Ions and its Applications

Tahir Savran, Journal of Fluorescence, (2024), DOI: 10.1007/s10895-024-03702-z

Oxidative stress is a trigger for many diseases and occurs with the unstable hypochlorite (ClO), known as one of the reactive oxygen species (ROS) in organisms. Then, HOCI is acknowledged as an oxidizing species that eliminates a variety of environmental pollutants. Hence, the development of novel methodologies for the selective and precise identification of HOCl/ ClO is considered to be of utmost importance. In this study, the design, characterization, and applications of a fluorene-based fluorescent probe (FHBP) dependent on the ESIPT mechanism with a "turn-on" response for the sensitive/selective determination of ClO against other competing samples were reported. The experimental results indicated that the detection limit for ClO could be quantitatively determined by the probe to be 8.2 × 10−7 M. The binding constant of the probe FHBP with ClO was computed as 9.75 × 103 M− 1. In addition, the response time of FHBP was appointed to be 30 s, indicating a rapid reaction with ClO. It has also been demonstrated that this probe can be successfully used for the detection of ClO on filter papers, TLC sheets, cotton swabs, and real samples.