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Utilizing PGSTE diffusion measurements for molecular weight determination by benchtop NMR In a previous application note, we discussed how the molecular weight of two PDMS polymers can be measured via end-group analysis in 1D ²⁹Si-NMR. Building on that discussion, this study focuses on the use of diffusion NMR spectroscopy to determine the molecular weight of …

Rapid molecular weight determination of Polydimethylsiloxanes (PDMS) Read More »

Reduce the quantification time of 13C 1000 times by including polarization transfer and indirect detection. The most common approach to quantifying materials by NMR utilizes 1H NMR spectra, which combines high intrinsic sensitivity with straightforward, inherently quantitative signal integrals. In complex formulations and reaction mixtures, however, 1H spectra often suffer from peak overlap, which limits …

q-HSQC for Sensitivity-Enhanced 13C Quantitation Read More »

Maximizing signal-to-noise ratio per measurement time by using the advantages of magnetization transfer in 29Si–1H-HMBC spectra. In a previous application note we discussed how the copolymer composition and average molar mass of poly(dimethylsiloxane-co-methylhydrosiloxane), trimethylsilyl terminated (Figure 1) can be determined by 29Si-DEPT NMR spectra and using a well characterized sample of the same substance as …

q-HMBC for Sensitivity-Enhanced 29Si Quantitation Read More »

Curcumin is a bright yellow polyphenolic compound produced by the turmeric (Curcuma longa) plant, a member of the ginger family, which has varied applications, such as an antioxidant, a natural dye or colouring and flavouring agent. The molecule exists in an equilibrium of keto and enol form (Figure 1), with the former preferred in water …

Spectrum of the Month: 1H J-res of Curcumin Read More »

Introduction Recent advances in laboratory automation and analytical instrumentation have opened new possibilities for accelerating reaction development, particularly in flow chemistry. Among these, the integration of real-time analytics with intelligent process control has enabled the emergence of self-optimizing reactor systems. These setups are capable of autonomously exploring reaction conditions to maximize the performance of the …

Automated optimization of a flow reactor by using Bayesian algorithms and Inline NMR Monitoring Read More »

Figure 1. 1H-15N HSQC of 15N-labelled GB1 measured in 9h on a Spinsolve at 80 MHz. The 2D spectrum shows the assignment of the signals for the different amino acids.  The structure of the GB1 protein is shown on the top left. Protein structures and their interactions with other proteins or small molecules such as …

App Note – 1H-15N HSQC of GB1 protein measured at 80 MHz Read More »

Naringin is a naturally occurring flavonoid that is responsible for the characteristic bitter flavour in citrus fruits like grapefruit and pomelo. Structurally it is an O-glycoside of naringenin (Figure 1). Figure 1. Structures of naringenin (left) and naringin (right) Although the structure of naringin is quite complex for a small molecule, the NMR spectra obtained …

Spectrum of the Month: 1H,1H COSY of Naringin Read More »

Introduction In this application note we show how benchtop NMR can be used to quantify kaurenoic acid, a diterpene with antibacterial activity, in Copaiba extract, a traditional Brazilian remedy for ailments such as rheumatism, ulcers and respiratory diseases, obtained from the perforation of the trunk of Copaiferea species. NMR Results and Discussion The quality of …

Kaurenoic Acid Can Be Quantified in Copaiba Extracts by 1H,13C HSQC. Read More »

Fast Analysis of Polysiloxanes by Benchtop NMR In a previous blogpost we discussed how copolymer composition can be determine by proton NMR (Blogpost). Polysiloxanes are often used as lubricants and the copolymer ratio has influence on the properties of the polymer, especially on the viscosity and the lifetime, and therefore a thorough characterization is important. …

Determine copolymer composition and average molar mass of polysiloxanes without sample preparation by using 29Si-DEPT spectra. Read More »

Application of the Spinsolve benchtop NMR in Structural Biology Figure 1. Cartoon representation of bovine serum albumin3,4 and dioxane. The grey circles represent the radius of hydration, a spherical approximation of size plus the excluded water volume. The size of biomacromolecules in solution is useful in basic structural biology research, as well as to characterize …

Determining the Protein Radius of Hydration by Diffusion NMR Read More »